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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Electrophoresis. 2018 Dec 27;40(4):571–581. doi: 10.1002/elps.201800417

Figure 2.

Figure 2.

Comparison of NTA-determined particle population characteristics following the various isolation methodologies. All values were normalized to number of particles (exosomes/particles) derived from 50 mL of starting cell culture. Reported values are averages from triplicate isolations. (A) EVs isolated via differential centrifugation. (B) EVs isolated via Qiagen exoEasy Maxi Kit. (C) Cell culture media cleared of cells and large debris via centrifugation, followed by exosome isolation via PET C-CP HIC. (D) Cell culture media cleared of cells and large debris via filtration, followed by exosome isolation via PET C-CP HIC.