TABLE 6.
Sequences of primers and probes used for assays
| Species/serogroup (assay name) | Primer/probe | Sequencea (5′–3′) | LODb | Reference |
|---|---|---|---|---|
| L. pneumophila | Lpneu F1 | GGAATTACTGGGCGTAAAGG | 10 target copies/reaction | 16 |
| Lpneu R1 | GAGTCAACCAGTATTATCTGACCG | |||
| Lpneu P1 | FAM-AAGCCCAGGAATTTCACAGAT-BHQ | |||
| L. pneumophila Sg1 | P65 F1 | CAAAGGGCGTTACAGTCAAACC | 10 target copies/reaction | 37 |
| P66 R1 | CAAACACCCCAACCGTAATCA | |||
| SG1P | FAM-TCTTGGGATTGGGTTGGGTTA-BHQ | |||
| M. avium | MAF | GGGTGAGTAACACGTGTGCAA | 10 target copies/reaction | 38 |
| MAR | CCAGAAGACATGCGTCGTGA | |||
| MAP | FAM-TGCACTTCGGGATAAGCCTGGGAAA-TAMRA | |||
| M. intracellulare | MIF | GGGTGAGTAACACGTGTGCAA | 10 target copies/reaction | 38 |
| MIR | CCACCTAAAGACATGCGACTAAA | |||
| MIP | FAM-TGCACTTCGGGATAAGCCTGGGAAA-TAMRA | |||
| M. abscessus | MAbF | CGATAGAGGACTTCGCCTACCG | 10 target copies/reaction | 39 |
| MAbR | TCGAGCACGTAAACTCCCTTTC | |||
| MAbP | FAM-CACTGACCGAACATCTATCCCGC-BHQ |
a
FAM, 6-carboxyfluorescein; BHQ, black hole quencher; TAMRA, 6-carboxytetramethylrhodamine.
b
LOD, limit of detection; the lowest target copy number per reaction giving a Cq of <40 in 6 of 6 independent qPCRs.