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. 2019 Nov 20;10:2621. doi: 10.3389/fimmu.2019.02621

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Copyright © 2019 Pizzagalli, Latino, Pulfer, Palomino-Segura, Virgilio, Farsakoglu, Krause and Gonzalez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutrophils phagocyte and transport influenza virus. (A) Schematic drawing (left) of an electron micrograph (right) showing neutrophils in the SCS phagocytizing UV-inactivated influenza virus at 2 h p.v.. (B) Flow cytometric analysis indicating the percentage of neutrophils positive for influenza vaccine (DiO labeled, left) and the corresponding MFI analysis (right). (C) Intravital 2-photon micrograph showing a LysM-GFP neutrophil (green) phagocytizing DiD labeled UV-PR8 (red) (left). Intravital 2-photon timelapse showing the correlation between the tracks from a UVPR8-positive vesicle and the neutrophil (right). (D) Distance of the viral particles from the cell centroid (black line), compared to the cell radius (red line). Results are given as mean ± SD. ns p > 0.05; *p < 0.05; **p < 0.01.