Table 3.

PI3K/Akt signaling related genes

Gene symbol	Probeset ID	CpG location	p-value (Ob vs. L)	Methylation difference (Ob − L)	Probeset ID	p-value (Ob vs. L)	Fold change
BCL2	cg07111678a	TSS1500	0.04	−0.33	203685_at	0.04	2.5
JAK1	cg25020373a	TSS200	0.01	−0.70	240613_at	0.03	2.4
MAP3K8	cg03807314b	TSS200	0.04	−0.19	235421_at	0.04	1.9
PPP2R1B	cg09085632a	TSS200	0.04	5.62	222351_at	0.02	−2.3
PPP2R2C	cg23195028b	Body	0.01	3.31	223573_s_at	0.01	−4.6
PPP2R3A	cg24636867a	TSS200	0.04	−0.34	207749_s_at	0.02	2.6
PPP2R5Cc	cg18997701b	Body	0.02	−0.29	1557718_at	0.01	2.2
PTEN	cg08602305a	5′UTR	0.03	−0.31	233254×_at	0.02	6.1
SYNJ2	cg03682872a	TSS200	0.03	−0.35	216180_s_at	0.04	1.5
THEM4	cg03147210a	TSS200	0.05	−0.52	229253_at	0.03	1.7
TFAMc	cg03859893b	TSS200	0.05	−1.02	238443_at	0.05	2.5

Data are presented as mean ± SD

Methylation Difference:  % Methylation (Ob)- % Methylation (L); values for  % methylation of groups are located in Additional file 4: Table S4. Significant probes from global DNA methylation analysis and global gene expression analysis were overlaid for matching genes; Probeset ID represents a single gene that may contain multiple methylation (CpG) sites. IPA Canonical Pathway tool identified PI3K/AKT Signaling as significantly overrepresented in our significant gene list

^aInfinium I probe design

^bInfinium II probe design

^cIndicates genes validated via qPCR. Gene names are located in Additional file 4: Table S4