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. 2019 Nov 27;16:239. doi: 10.1186/s12974-019-1651-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The expression of NLRP3 and caspase-1 in each group at different time points. a, b NLRP3 and caspase-1 in the cortex harvested at specific time points after CLP. The protein levels were normalized to those of tubulin protein and are shown as relative arbitrary units. c Fluorescence of NLRP3 and caspase-1 in the cortex at specific time points after CLP. Three-color staining for anti-NLRP3 antibody (green), anti-caspase-1 (red), and nucleus (blue), the protein volume and coincidence rate are represented by histograms. Scale bar = 40 μm. Values are expressed as mean ± SEM (n = 6 in each group; *P < 0.05 vs. sham group, in the corresponding group)