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Single soleus fibers run on SDS-PAGE gel to identify fiber types. Each lane is the MHC extracted from a single isolated muscle fiber. (A) At 3 weeks of age, many of the soleus fibers are I/IIA hybrids (asterisks). (B) By 12 weeks, the majority of fibers (all but one in this example) are type I. This example shows the transformation of the hybrid fibers into pure type I fibers. Gels of individual, isolated muscle fibers were used for the quantitative analyses of fiber type proportions.
