Fig. 6.

Local delivery of Atsttrin improves neuroinflammation and apoptosis in PGRN-deficient mice after SCI. a BMS was used to evaluate the therapeutic effect of hydrogel/Atsttrin in both WT and PGRN-deficient mice. Asterisk indicates p < 0.05 between Grn^−∕−-SCI-gel/PBS and Grn^−∕−-SCI-gel/Atsttrin groups. b At day 7 after SCI, total protein was extracted from injured spinal cord of PGRN-deficient mice and iNOS, p-p65, p65, and GAPDH were detected by Western blotting. Quantification of iNOS/GAPDH (c) and p-p65/p65 (d) in (b). n = 3 for each group. e At day 7 after SCI, total protein was extracted from injured spinal cords of PGRN-deficient mice and Bax, Bcl-2, and GAPDH were detected by Western blotting. Quantification of Bax/GAPDH (f) and Bcl-2/GAPDH (g) in (e). n = 3 for each group. *p < 0.05, **p < 0.01 for indicated groups, ns, not significant