Table 1.
Summary of sequencing quality
| Sample ID | Raw reads | Clean reads | Clean bases | Error (%) | Q20 (%) | Q30 (%) | GC (%) |
|---|---|---|---|---|---|---|---|
| Dm_R1 | 104,974,434 | 103,116,856 | 15.24Gb | 0.0126 | 98.12 | 94.61 | 46.41 |
| Dm_R2 | 112,417,410 | 110,298,744 | 16.38Gb | 0.012 | 98.43 | 95.25 | 46.63 |
| Dm_R3 | 93,916,312 | 91,971,512 | 13.59Gb | 0.0125 | 98.22 | 94.7 | 46.35 |
| Dm_S1 | 95,188,100 | 93,364,948 | 13.79Gb | 0.0129 | 97.99 | 94.31 | 46.49 |
| Dm_S2 | 83,861,418 | 81,881,046 | 12.08Gb | 0.0127 | 98.12 | 94.44 | 46.1 |
| Dm_S3 | 95,368,208 | 92,929,138 | 13.69Gb | 0.0128 | 98.05 | 94.27 | 46.07 |
| Dm_L1 | 84,551,998 | 83,097,662 | 12.29Gb | 0.0126 | 98.13 | 94.64 | 46.66 |
| Dm_L2 | 77,755,976 | 75,885,672 | 11.18Gb | 0.0127 | 98.11 | 94.43 | 46.36 |
| Dm_L3 | 80,621,980 | 78,905,586 | 11.64Gb | 0.0127 | 98.12 | 94.45 | 46.15 |
| Total | 828,655,836 | 811,451,164 | 119.89Gb |
(1) Q20: percentage of bases with a Phred value > 20; Q30: percentage of bases with a Phred value > 30. (2) Error (%): Base error rate. (3) GC (%): percentage of bases G and C number in the total number of bases