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. 2019 Nov 27;7:326. doi: 10.1186/s40425-019-0817-4

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — GDNPs are efficiently taken up by macrophages. a Confocal images (top) and FACS quantitative analysis (bottom) of DiI-labelled GDNPs (10 μg/ml) taken up by BMDM, B16F10, CT26 and HEK293T cells. Cells were incubated with DiI-labelled GDNPs for 12 h (Scale bar = 20 μm). b Quantitative flow cytometry analysis of DiI-labelled GDNPs taken up by BMDM (F4/80-FITC) at different time points. c Uptake efficiency was quantified by flow cytometry (n = 4). d In vivo biodistribution of GDNPs was determined by scanning mice that received an i.p. injection of DiR-labelled GDNPs. The main organs of the treated mice were examined. e In vivo FACS quantitative analysis of spleen cells uptake of DiI-labelled GDNPs administrated i.p. injection