Fig. 5.

Cyp27b1 gene knockout aggravates BLM-induced pulmonary interstitial fibrosis. All animals were fed with standard fodder. All animals except controls were injected with BLM (1.5 mg/kg) through the trachea. All animals were sacrificed 2 weeks after BLM. a and b Pulmonary histopathological injury and inflammation were evaluated by H&E. a A representative picture. Original magnification: 200×. b Pulmonary pathological scores were evaluated. c Inflammatory cells in the lungs. d and e Collagen deposition was evaluated using Sirius red staining. d A representative picture. Original magnification: 200×. e The area of collagen deposition was calculated. All data were expressed as means ± SEM (N = 6). **P < 0.01, *P < 0.05