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. 2019 Nov 21;10:2702. doi: 10.3389/fimmu.2019.02702

Figure 2.

Figure 2

Apoptosis is induced in DCs after 4 days of activation with LPS and IFNγ. (A) Morphology of 1- and 4-day activated DC1s (400X magnification). Four-day activated DC1s exhibit clear signs of undergoing apoptosis such as cell shrinkage and increased amounts of cell debris. Photographs are representative of six donors (n = 6). (B) Apoptotic DCs were detected by staining with Annexin V and 7-AAD and percentages of live, pre-apoptotic, and dead cells is shown. The data is representative of three independent experiments with four donors (n = 4). (C) Viability of DCs as a LIVE/DEAD Fixable Near-IR Stain negative percentage of FSC and SSC gated cells. Four-day activated DCs were cultured in the precense of specific inhibitors targeting TAM receptor signaling (iTAM) and/or efferocytosis (iEffero). (D) IL-10 and (E) IL-12p70 was measured in the supernatants and (F) the viability of DCs was measured with LIVE/DEAD Fixable dye. Cumulative data are shown from two independent experiments with four donors (n = 4). Bars represent mean + standard deviation. Two-way ANOVA with Tukey's post-hoc or Dunnett's multiple comparisons test was performed on non-transformed data to compare groups (****p ≤ 0.0001, **p ≤ 0.01, and ns, not statistically significant).