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. 2019 Nov 21;25:8820–8835. doi: 10.12659/MSM.919721

Figure 3.

Figure 3

SIRT1 may be a miR-155 target. Alignment between the miR-155 seed sequence and putative targeting sites in the SIRT1 3′UTR represented in red and green letters, respectively. (A) Sequence of human miR-155 and the predicted binding sequence with miR-155 within SIRT1 3′ untranslated regions (3′ UTRs) depend on the online prediction tool Target Scan are shown. (B, C, F) Luciferase reporter constructs containing F1-R1, mut F1-R1 T1, and F5-R2 sites of SIRT1 gene co-transfected with miR-155 mimics and the luciferase activities were assayed (n=5, ** P<0.01 vs. control). (D) All potential target sides within SIRT1 3′ UTRs we predicated are shown. (E) Sequence of human miR-155 and potential target binding sequences with a mismatch base pairing range from T1 to T4 are shown.