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. 2019 Nov 27;14:214. doi: 10.1186/s13014-019-1418-6

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — γH2AX foci in isogenic FaDu, FaDu-RR and 2A3 cells after 5 Gy irradiation. a A median number of γH2AX foci in isogenic cells determined by γH2AX immunofluorescence at various recovery times after irradiation. Box plots are median values with 25th and 75th percentile with 10th and 90th percentiles as bottom and top whiskers. ** indicates a significant difference in γH2AX foci number between isogenic FaDu, FaDu-RR, and 2A3 cells; * indicates a significant difference in γH2AX foci number. b The fraction of γH2AX-positive isogenic cells at various recovery times after irradiation. Bars are AM ± SEM from three independent experiments. * indicates a significant difference in the fraction of in γH2AX-positive cells compared to control non-irradiated cells. c Kinetics of γH2AX foci resolution in isogenic cells. Experimental data were fitted to a two-phase exponential decay equation. Symbols are the median values of pooled data from three independent experiments