Table 4.
DNA damage signaling gene expression in 5 Gy-irradited radioresistant FaDu-RR cells and 5 Gy-irradiated radiosenstive 2A3 cells compared to 5 Gy-irradiated parental FaDu cells
| Genea | FaDu-RRb | P valuec | 2A3d | P valuec | DNA damage signaling pathways |
|---|---|---|---|---|---|
| APEX1 | 1.89 | 0.001 | BER | ||
| ATM | 1.87 | 0.01 | ATM/ATR signaling, DSB repair, apoptosis, cell cycle | ||
| BAX | −2.49 | 0.006 | apoptosis | ||
| BBC3 | −3.65 | 0.003 | apoptosis | ||
| BLM | −1.69 | 0.02 | −7.86 | 0.000 | DSB repair |
| BRIP1 | −2.35 | 0.02 | Other repair genes | ||
| CDC25A | −1.59 | 0.006 | ATM/ATR signaling, cell cycle | ||
| CHEK2 | −1.65 | 0.02 | ATM/ATR signaling, other repair genes, apoptosis, cell cycle | ||
| CIB1 | −5.60 | 0.001 | Other repair genes, apoptosis | ||
| FANCA | −2.02 | 0.001 | Other repair genes | ||
| HUS1 | −1.65 | 0.001 | ATM/ATR signaling, DSB repair | ||
| MDC1 | −1.66 | 0.000 | DSB repair, cell cycle | ||
| MLH1 | 1.51 | 0.02 | DSB repair, mismatch repair | ||
| MPG | −1.85 | 0.04 | BER | ||
| NBN | −1.83 | 0.02 | DSB repair | ||
| NTHL1 | −1.54 | 0.02 | BER | ||
| PNKP | −2.77 | 0.000 | NER | ||
| RBBP8 | 1.53 | 0.02 | ATM/ATR signaling, other DNA repair genes | ||
| RNF168 | −2.77 | 0.001 | ATM/ATR signalling, other DNA repair genes | ||
| RPA1 | −1.70 | 0.04 | NER, DSB repair | ||
| SMC1A | −1.53 | 0.005 | ATM/ATR signalling, other DNA repair genes | ||
| SUMO1 | −2.73 | 0.002 | Other DNA repair genes | ||
| TP73 | −2.12 | 0.04 | Mismatch repair, apoptosis, cell cycle | ||
| XPA | 2.28 | 0.004 | −1.54 | 0.03 | NER |
| XRCC3 | −2.20 | 0.01 | other DNA repair genes |
a Gene symbol according to HUGO Gene Nomenclature Committee; b Fold regulation in gene expression in 5 Gy-irradiated FaDu-RR cells in comparison to 5 Gy-irradiated FaDu cells. Positive fold regulation values indicate gene over-expression, negative fold regulation values indicate gene under-expression; c The P values were calculated based on a Student’s t-test of the replicate 2(− ΔΔ CT) values for each gene in the 5 Gy-irradiated and non-irradiated cells, and differences in gene expression were considered significant for P values less than 0.05. d Fold regulation in gene expression in 5 Gy-irradiated 2A3 cells in comparison to 5 Gy-irradiated FaDu cells. DSB double-strand break, BER base excision repair, NER nucleotide excision repair