. Author manuscript; available in PMC: 2019 Nov 28.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jun 19;1092:447–452. doi: 10.1016/j.jchromb.2018.06.040

Table 2:

Summary of the linear equation for valacyclovir (VACV) and acyclovir (ACV) over the concentration range from 2 to 5000 nM in mouse and human plasma. Better accuracy was achieved when the range was separated into two small range. The weighing factor of 1/x² was used. Y is the peak area ratio of the analyte to internal standard and x the concentration of the analyte.

Compound	Conc. range (nM)	Mouse plasma (n= 5)	Human plasma (n= 5)
ACV	2–200	y = 0.0041 (± 0.00031) x + 0.027 (± 0.0034), R² > 0.99	y = 0.0042(± 0.00013) x + 0.0035(± 0.00085), R² > 0.99
ACV	200–5000	y = 0.0027(± 0.000085) x + 0.30 (± 0.017), R² > 0.98	y = 0.0026(± 0.000063) x + 0.34(± 0.032), R² > 0.99
VACV	2–200	y = 0.0030(± 0.000070) x + 0.0087(± 0.0014), R² > 0.99	y = 0.0031 (± 0.000090) x + 0.0034(± 0.00057), R² > 0.99
VACV	200–5000	y = 0.0023(± 0.000052) x + 0.17(± 0.018), R² > 0.99	y = 0.0022(± 0.000071) x + 0.21 (± 0.024), R² > 0.99