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. 2019 Nov 26;9(1):1696517. doi: 10.1080/20013078.2019.1696517

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — MP-sEV labelling by chimeric gLuc proteins and stability in serum. (a) Schematic workflow of MP-sEV labelling with chimeric gLuc proteins. (b) The luciferase activity per sEV protein amounts of MP-sEV incubated with approximately 5–7 × 10⁹ RLU of gLuc-LA, gLuc-Lys or gLuc-PFG. The results are expressed as the percentage relative to gLuc-LA. (c) Time-course of gLuc activity for gLuc-LA-, gLuc-PFG- or gLuc-Lys-labelled MP-sEVs incubated with 10% mouse serum in PBS at 37°C. (d–f) SEC analysis of (d) gLuc-LA-, (e) gLuc-PFG- or (f) gLuc-Lys-labelled MP-sEVs incubated with 10% mouse serum in PBS at 37°C for the indicated time periods.