Table 1.

Primer sequences for site directed mutagenesis.

Mutation	Orientation	Primer sequence
K112A	Forward	5′-GCC ACC CAT CTC CTG CTT CAG CGC GCC CAT GTT GAA-3′
	Reverse	5′-TTC AAC ATG GGC GCG CTG AAG CAG GAG ATG GGT GGC-3′
L105A; Y107A	Forward	5′-GCC CAT GTT GAA GGC GAA GGC GGC CCC GGC CGT-3′
	Reverse	5′-ACG GCC GGG GCC GCC TTC GCC TTC AAC ATG GGC-3′