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Determining efficiency of REIUS method in removing cytokines. (a,b) comparison between REIUS and REIUS flow-through (REIUS FT). (c,d) direct comparison between SEC alone and REIUS method and the presence of representative soluble factors (IFN-γ, IL-7, IL-8 and IL-10) in either 888-mel or 2183-Her4 supernatant isolates. REIUS method is capable of removing more soluble factors than SEC alone (n = at least 6).
