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The experimental procedures for each group of control, ICD, and ACD mice tested on the cheek. The same procedure was applied in experiments using skin from the calf with the exception that the calf was challenged with acetone or 1% SADBE only on days 8 and 9. ACD, allergic contact dermatitis; ICD, irritant contact dermatitis; SADBE, squaric acid dibutylester.
