Figure 6.

Effects of ACD and ICD on cytokine protein expression in the calf skin. Exemplary images of immunostaining for IL-1β (A), TNF-α(B), CXCR3 (C), and CXCL10 (D) obtained for ACD, ICD, and control mice (shown at magnifications of ×50, ×100, and ×200). ACD, allergic contact dermatitis; ICD, irritant contact dermatitis. Protein expression levels of IL-1β (E), TNF-α (F), CXCR3 (G), and CXCL10 (H) were obtained after the second challenge. *P < 0.05, **P < 0.01, error bars: SEM. n = 3 to 5 male mice/group.