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. 2019 Nov 22;10:2548. doi: 10.3389/fmicb.2019.02548

FIGURE 7.

FIGURE 7

Bacillus pumilus B12 increases alters L-lactate release from PLA when grown in different carbon sources. (A) Initial measurements of B. pumilus B12 grown in different primary carbon sources at a concentration of 55 μM taken after 48 h. Red bars indicate the addition of PLA, black bars indicate B12 grown in media only. All **** and ns above red bars indicates ANOVA ****p-value ≤ 0.0001; ns, not significant against media only control. **** above the line indicates p-value ≤ 0.0001 against glucose carbon source. These assays were performed with three biological and three technical replicates. (B) Optical density-based growth curves of B. pumilus B12 in the carbon sources taken over 48 h. Each growth curve represents twelve biological replicates. (C) Release of L-lactate from PLA normalized for growth of B. pumilus B12 after 48 h. ANOVA results ****p-value ≤ 0.0001 ns, not significant against standard glucose supplementation.