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. 2019 Nov 22;13:1247. doi: 10.3389/fnins.2019.01247

FIGURE 4.

FIGURE 4

Acid-induced currents in ASIC1b-expressing DRG neurons. (A) Representative current traces of ASIC-like currents in ASIC1b-expressing DRG neurons. Amiloride (100 μM) inhibited the acid-induced transient inward current (n = 5). (B) Representative current traces of acid-induced sustained currents in ASIC1b-expressing DRG neurons (n = 5). (C) Representative current traces of acid-induced sustained currents resistant to mambalgin-1 (1 μM). (D) Representative current traces of acid-induced sustained currents sensitive (n = 1) or resistant (n = 3) to 10 μM ruthenium red treatment. (E) Histograms of DRG neurons by size and acid-induced current types (biphasic, n = 81; sustained, n = 61). (F) pH sensitivity of DRG neurons with biphasic and sustained currents. Peak amplitudes were normalized to that induced by pH-5 artificial cerebrospinal fluid (ACSF) (biphasic, n = 10; sustained, n = 10). (G) The proportion of three subtypes of ASIC-like currents with different desensitization time constants (rapid, <300 ms; intermediate, 300–900 ms; and slow, >900 ms).