TABLE 2.
Electrophysiological properties of three types of ASIC1b-TdTomato(+) DRGs with different desensitization rates.
| Rapid (n = 42) τdesens < 300 | Intermediate (n = 15) τdesens300−900 | Slow (n = 10) τdesens > 900 | Rapid vs. intermediate | Rapid vs. slow | Intermediate vs. slow | Test | |
| AP threshold (mV) | −32.2 ± 1.2 | −35.7 ± 4.7 | −39.6 ± 1 | NS | P < 0.01 | NS | KW |
| AP rheobase (pA) | 678 ± 501.2 | 404.6 ± 64.2 | 316.5 ± 105.6 | NS | P < 0.01 | NS | KW |
| AP half-width (ms) | 1.3 ± 0.9 | 0.9 ± 0.1 | 0.8 ± 0.1 | NS | NS | NS | KW |
| AP overshoot (mV) | 33.7 ± 2.6 | 17.5 ± 3.5 | 24.5 ± 4 | P < 0.01 | NS | NS | ANOVA |
| RMP (mV) | −58.9 ± 0.6 | −51.9 ± 1 | −51.9 ± 0.8 | P < 0.0001 | P < 0.0001 | NS | ANOVA |
| Cell size (μm) | 39 ± 0.7 | 38 ± 1.5 | 40.9 ± 1.4 | NS | NS | NS | ANOVA |
| Membrane capacitance (pF) | 46.1 ± 4.9 | 34.3 ± 3.7 | 60.4 ± 9 | NS | NS | NS | KW |
| Membrane resistance (mΩ) | 248.1 ± 31.7 | 263.6 ± 41.8 | 363.7 ± 146.6 | NS | NS | NS | KW |
| Membrane time constant (ms) | 0.8 ± 0.1 | 0.6 ± 0.1 | 0.9 ± 0.1 | NS | NS | P < 0.05 | KW |
| Current amplitude (pA) evoked by pH5 acid | 3460 ± 387.9 | 4025 ± 480.6 | 4877 ± 778.3 | NS | NS | NS | KW |
| τdesens | 188.8 ± 10.2 | 556.7 ± 43.8 | 1127 ± 100.2 | P < 0.0001 | P < 0.0001 | NS | KW |
| Inhibition by MB-1 | 88% (8/9) | 100% (3/3) | 100% (2/2) | NS | NS | NS | F |
| Inhibition by PcTx1 | 33% (2/6) | 80% (4/5) | NA | NS | NA | NA | F |
| Inhibition by APETx2 | 55% (5/9) | 50% (1/2) | 0% (0/2) | NS | NS | NS | F |
Characterization of electrophysiological properties in three types of DRG neurons with different desensitization rate (τdesens; rapid, intermediate and slow) of biphasic current. KW, Kruskal–Wallis test followed by Dunn’s test; ANOVA, one-way ANOVA followed by Tukey’s test; F, Fisher’s exact test; RMP, resting membrane potential; NS, not significant; NA, not available.