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. 2019 Nov 29;4:51. doi: 10.1038/s41392-019-0086-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HOXB13 exhibits antitumorigenic properties in colon cancer cells. a The mRNA levels of HOXB13 in human colon cancer cell lines and a normal human colon mucosal epithelial cell line were compared. b Protein levels of HOXB13 were examined by western blotting. GAPDH was used as a loading control. c HOXB13 knockdown and overexpression were validated by western blot analysis. d, e A CCK-8 assay was performed to assess the proliferation of HCT116/sh-HOXB13 and RKO/HOXB13 OE cells. f–i A cell clonogenic assay was used to measure the capacity of HCT116/sh-HOXB13 and RKO/HOXB13 OE cells to form colonies. Data are presented as the mean ± SD. ****P < 0.001. A t-test was used for the statistical analysis. j, k Flow cytometry and data analysis were performed to examine apoptosis in RKO/HOXB13 OE cells