Fig. 4.

HOXB13 exhibits antitumorigenic properties in colon cancer cells in vivo. a–d Representative images of euthanized mice and xenograft tumors at day 30 after subcutaneous injection (n = 6). Tumor sizes are depicted as the tumor volume. e, g Representative images showing HE and Ki-67 staining of xenograft tumor tissues. Scale bars represent 50 μm. f, h Comparison of the proportion of Ki-67-positive HCT116/sh-HOXB13 (or RKO/HOXB13 OE) cells and the corresponding controls. i, j Apoptotic cells in xenograft tumors were detected with TUNEL assays. Data are presented as the means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001