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. 2019 Nov 28;9:17807. doi: 10.1038/s41598-019-54282-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stroma-corrected ZEB1 expression is inversely correlated with the abundance of multiple immune cell types and with immune cell recruitment to breast tumors. (A–C) Residual plot of ZEB1 expression and xCELL macrophage (A), Th1 cell (B) and mast cell (C) abundance corrected for stromal cell content. (D–F) Residual plot of ZEB1 expression and TIP-based estimation of T cell (D), natural killer cell (E) and macrophage (F) recruitment. (G) Immunohistochemistry measurement of monocyte percentage in tumor samples compared by samples with greater or less than median stroma-adjusted ZEB1 expression (median percentage 0%). (H) Immunohistochemistry measurement of lympocyte percentage in tumor samples compared by samples with greater or less than median stroma-adjusted ZEB1 expression (median percentage 1%).