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. 2019 Nov 28;9:322. doi: 10.1038/s41398-019-0662-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a An illustration of the mPFC boundary after cresyl violet staining. The area within the red lines was the mPFC. Scale bar = 1 mm. b An illustration of the method used to estimate the total volume of the mPFC. The points located inside the red line were counted. Scale bar = 1 mm. c Total volume of the mPFC in the nonstressed group, stressed group and stressed + running group (mean ± SD, n = 5 per group). *p < 0.05, **p < 0.01. mPFC medial prefrontal cortex.