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. 2019 Nov 28;9:322. doi: 10.1038/s41398-019-0662-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative pictures of immunohistochemical staining with anti-NG2 antibody in each subregion of the mPFC of the nonstressed rats, stressed rats and stressed + running rats. Scale bar = 40 μm. b Total NG2⁺ cell number in the mPFC in the nonstressed group, stressed group and stressed + running group (mean ± SD, n = 5 per group). c The protein expression of NG2 in the mPFC of each group rats was detected using western blot. d Semiquantitative analyses of the protein level of NG2 in the mPFC from each group (mean ± SD, n = 8 per group). e Representative pictures of immunohistochemical staining with anti-CNPase antibody in each subregion of the mPFC from the nonstressed rats, stressed rats and stressed + running rats. Scale bar = 40 μm. f Total CNPase⁺ cell number of mPFC in the nonstressed rats, stressed rats and stressed + running rats (mean ± SD, n = 5 per group). g The protein expression of CNPase in the mPFC of each group of rats was detected using western blot. h Semiquantitative analyses of the protein level of CNPase in the mPFC from each group (mean ± SD, n = 8 per group). **p < 0.01. PL prelimbic, IL infralimbic, ACC anterior cingulate cortices.