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. 2019 Nov 28;9:322. doi: 10.1038/s41398-019-0662-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative pictures of immunofluorescence staining with anti-Olig2 antibody and anti-NG2 antibody in the mPFC from the nonstressed group, stressed group and stressed + running group. Scale bar = 50 μm. b Quantification of Olig2⁺ cell number in the mPFC from the three groups of rats after immunofluorescence staining (mean ± SD, n = 5 per group). c Quantification of Olig2⁺/NG2⁺ cell number in the mPFC from the three groups of rats after immunofluorescence staining (mean ± SD, n = 5 per group). d The percentage of Olig2⁺/NG2⁺ cells of the Olig2⁺ cells in the mPFC from three groups of rats after immunofluorescence staining (mean ± SD, n = 5 per group). e The protein expression of Olig2 in the mPFC from each group of rats was detected using western blot. f Semiquantitative analyses of the protein level of Olig2 in the mPFC from each group (mean ± SD, n = 8 per group). *p < 0.05, **p < 0.01. mPFC medial prefrontal cortex.