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. 2019 Nov 28;9:17762. doi: 10.1038/s41598-019-54166-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Diurnal gene expression levels at four hour intervals over two seasons. Gene expression for apple Gigantea (GI) and BBX1 in apple leaf tissue and fruit skin tissue labelled, all other genes are fruit skin results. Other genes tested were light-associated COP1, HY5, and UVR8, temperature-linked genes CBF2 and HSFA2a and BBX genes 15, 33, and 51 from BBX group IV over two seasons. Expression results were normalised to Actin and error bars are SE of four technical replicates. Daylight hours were from 0640 h to 2040 h for season 1 and from 0700 h to 2000 h for season 2, rounded to the nearest 10 min. Black bars indicate night.