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. 2019 Nov 28;9:17762. doi: 10.1038/s41598-019-54166-2

Figure 7.

Figure 7

Characterisation of 35S:BBX1 transgenic lines. (A) Images of representative apples from each of the lines. (B) Cyanidin concentration in fruit skin for the transgenic lines compared with ‘Royal Gala’ control (RG). Cyanidin concentrations reported are the sums of cyanidin 3-O-galactoside, cyanidin 3-O-glucoside, and cyanidin 3-O-arabinoside in µg/g FW. Cyanidin 3-O-galactoside accounted for over 90% in every sample tested. (C) Gene expression of BBX1 in the 35S:BBX1 transgenic lines compared with that in ‘Royal Gala’ control fruit (RG). Results are average expression of three biological replicates and four technical replicates relative to Actin. Error bars show SE of four biological replicates. Differences between averages were statistically analysed using ANOVA and pairwise comparison performed using Fisher’s LSD test at the 5% significance level.