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. 2019 Nov 28;9:17798. doi: 10.1038/s41598-019-54167-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Encapsulated NSC Increase Endogenous Neural Stem Progenitor Cell Proliferation and Differentiation. (A) Confocal image of injected microbeads position into the striatum relatively to the lateral ventricle (LV). Few GFP-tagged NSC were observed migrating towards SVZ two weeks after injection, however at day 3, no GFP-tagged NSC were founded in the SVZ. (B) Confocal images staining for Ki67 and Sox2 stained tissue sections of control (upper panel), encapsulated NSC (middle panel) and encapsulated NSC and EC (lower panel) groups, quantifying co-localization of proliferating NSC (Ki67⁺/Sox2⁺) in SVZ tabled in (D). (C) Confocal images staining for DCX and Hoechst to visualize endogenous NSC maturation in SVZ, quantifying endogenous DCX⁺ cells tabled in E. Error bars represent SEM. Three different SVZ regions were selected from each brain tissue for the analyses (n = 3-5). **p < 0.01 determined by one-way ANOVA with multiple comparisons. Scale bar (25 µm) representative of all images.