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. 2019 Nov 28;9:17789. doi: 10.1038/s41598-019-54454-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Preparation for standard tapes for calibration curves. Pink square areas of D-squame tapes are coated with the NMF mixture solutions with different concentrations. (A pink color density represents the concentration of the NMF mixture.) Then, the blue square areas are coated with the SIL-IS mixture solution with a concentration of 1 nmol/cm² on a tape. After that, those tapes are set on the prism and measured by DART-MS.