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. 2019 Nov 28;2:440. doi: 10.1038/s42003-019-0686-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — ToxT_ENV256 K231A is less sensitive to oleic acid and has reduced thermostability. Electrophoretic mobility shift assays showing the effect of oleic acid on DNA binding of ToxT_ENV256 wild-type (a) and K231A (b). All lanes contain 9 nM DIG-labeled 84 bp segment of dsDNA containing the ToxT-binding sites from the tcpA promoter DNA and 0.78 μM ToxT_ENV256. The concentration of oleic acid in each lane is shown. c Thermostability of wild-type (black) and K231A (gray) ToxT_ENV256 as determined by circular dichroism.