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. 2019 Nov 28;9:17810. doi: 10.1038/s41598-019-54306-8

Figure 5.

Figure 5

Bßglucans attenuate the vascular smooth muscle cell inflammation and osteogenic differentiation induced by LPS. Gene expression of nSMase2 (a), TNFα (b), ADAM17 (c), α-actin (d), Runx2 (e) and Osterix (f) in A7r5 cells exposed to control medium (CONTROL), 100 μg/mL of Bßglucans (β-GL), 5 µg/mL of LPS (LPS) or the combination of 100 μg/mL of Bßglucans and 5 µg/mL of LPS (LPS + ß-GL) for 16 hours. Bars and error bars represent mean ± SD from three independent experiments, each performed in triplicate per experimental condition. R.U.: relative units. **p < 0.01 and ***p < 0.001 vs. Control, #p < 0.05 and ###p < 0.001 vs. LPS.