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. 2019 Nov 29;4:50. doi: 10.1038/s41392-019-0078-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — SerRS directly interacts with Shelterin POT1. a Immunofluorescent staining to show the colocalization of SerRS (green) and POT1 (red) in the nucleus of HeLa VST cells. Scale bars represent 5 μm. b Flag-tagged SerRS (Flag-SerRS) and V5-tagged POT1 (V5-POT1) vectors were transfected into HeLa cells, and immunoprecipitation (IP) was performed with the indicated antibodies. Cell lysates and immunoprecipitated proteins were analyzed by immunoblotting (IB) with the indicated antibodies. c HeLa VST cell lysates were immunoprecipitated with a POT1 antibody or a nonspecific IgG and analyzed by immunoblotting with the indicated antibodies. d A GST tag was fused with full length (FL) SerRS or the tRNA-binding domain (TBD), catalytic domain (CD) and unique to SerRS domain (UNE-S) of SerRS (upper panel). The GST-fused SerRS proteins were used to pull down purified His6-tagged POT1, and they were analyzed by immunoblot with anti-His6 antibody and anti-GST antibody (lower panel). e GST pull-down assays showed the direct interaction between SerRS and different domains of POT1. Full-length (FL) POT1 and its domains were fused with GST at the N-terminus to pull down purified His6-tagged SerRS. OB1, OB2 and OB3 stand for oligonucleotide/oligosaccharide DNA-binding domains 1, 2, and 3; HJR stands for Holliday junction resolvase-like domains