Fig. 1.

Design of 2D wireframe scaffolded DNA origami objects with DX and 6HB edges. a Arbitrary target geometries can be specified as input in one of two ways: Boundary and internal design, specifying the complete internal and boundary geometry using piecewise continuous lines; or Boundary design, defining only the border of the target object, with the internal mesh geometry designed automatically. b DX-based 2D wireframe scaffolded DNA origami objects published previously, PERDIX²⁶. Each wireframe edge is connected covalently to its neighboring edges by one scaffold and one staple crossing. c 6HB-based 2D wireframe scaffolded DNA origami, METIS. This 6HB geometry forms three layers connected with scaffold double-crossovers. Each wireframe edge is connected covalently to its neighboring edges by three scaffold and staple crossings. d The target geometry presents six DNA duplexes per wireframe edge and forms closed loops with geometrically allowable scaffold double-crossovers between them. The dual graph of the loop-crossover structure is obtained by converting each closed scaffold loop to a node and each possible scaffold double crossover connecting them to an edge. The minimum spanning tree of the dual graph was then determined and inverted, defining the DNA scaffold routing.