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. 2019 Nov 28;10:5419. doi: 10.1038/s41467-019-13457-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Controlled arm angles for a triangle, square, hexagon, and octagon without internal mesh. Target geometries without internal mesh are designed with the radius of the circumscribed circle of 25 nm that corresponds to the triangle a with 128-bp edge length and octagon b with 74-bp edge length, corresponding to approximately 43 and 25 nm, respectively. Comparison of DX- and 6HB-based DNA origami objects with (left) AFM, (middle) TEM, and (right) histogram analyses of the distributions in angles. c TEM and histogram analysis of the distributions in angle of the 6HB-based DNA square of 105-bp edge length. d TEM for the 6HB-based DNA octagon of 57-bp edge length. The top zoomed-in image on each panel is the representative object that is the most deformed and the image below represents the object that is the least deformed in shape. Scale bars in zoom-in and zoom-out AFM and TEM images are 50 and 200 nm, respectively. Source Data are available in the Source Data file.