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. 2019 Nov 28;10:5425. doi: 10.1038/s41467-019-13489-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Implantation is aberrant in Scrib^f/fPgr^cre/+ mice. a Histology of day 5 of implantation sites in each genotype. Scale bar, 200 μm. Arrowheads indicate the location of embryos. b 3D imaging of day 5 implantation sites in Scrib^f/f, Scrib^f/fLtf^cre/+, and Scrib^f/fPgr^cre/+ females. Images of E-cadherin immunostaining, segmented, and 3D rendered images of day 5 implantation sites in each genotype show the aberrant crypt-gland structure in Scrib^f/fPgr^cre/+ females. Images were generated by a Nikon A1R Multiphoton Microscope with LWD 16× objective with 3 µm Z-stack. Scale bar, 200 μm. Asterisks indicate the location of blastocysts. Each image is a representative of at least three independent experiments. M mesometrial pole, AM antimesometrial pole, le luminal epithelium, ge glandular epithelium, st stroma.