Figure 4.
Placental oxidative stress and apoptosis in young versus aged dams. Images of entire immunoblots showing oxidatively damaged proteins (A) with protein quantitation (B) in females and males. Western blot images of antioxidant enzymes GPX1, SOD2 and CAT (C) in female and male placentas with abundance of proteins adjusted for Ponceau S staining (D). Representative images of sections showing cleaved caspase 3 immunohistochemistry in the junctional zone (E) and labyrinthine zone (F) with abundance quantified (G,H, respectively) in female and male placentas. Representative images of sections showing Tunel staining (I) with abundance quantified in the junctional zone (J) in female and male placentas. Data are from n = 4 placentas per group, each from different litter and presented as mean + SEM values. Jz: junctional zone, Lz: labyrinth zone. Significant difference between young and aged dams were determined by unpaired student t test, *p < 0.05, **p < 0.01.