Fig. 2.

Ectopic expression of PCDH17-induced apoptosis and autophagy in HCT116 and SW480 cells. a PCDH17 expression in stably transfected cells as confirmed by RT–PCR and western blot. b, c Effect of ectopic expression of PCDH17 on the viability of CRC cells. Cell viability was measured via the CCK-8 assay after 5-FU treatment. Data are presented as the means ± standard deviations (SDs). The experiments were performed in triplicate. d Western blot analysis was performed to determine the expression of apoptosis and autophagy proteins in CRC cells transfected with empty vector or PCDH17 siRNA.