Figure 1.

Examples of artifact in both fluorescence and brightfield captured images. Images (A–D) are examples of multiplex IF images containing different types of artifacts. These images were taken from slides labeled with Pan-cytokeratin (green), DAPI (blue), CD3 (yellow), and CD8 (red). (A) Higher intensity of Pan-cytokeratin on the right region than the left as defined by the dotted white line. (B) White arrows point to high intensity regions in the DAPI channel artificially produced during imaging. (C,D) White arrows show tears and folds in the tissue that result in out of focus and fluorescence artifacts. Images (E–H) contain examples of artifacts from brightfield captured images labeled with H&E (E,G,H) or Verhoeff's elastic stain (F). (E) Black arrow highlights foreign object under coverslip. (F) Red arrow highlights out of focus region. (G) Black arrow shows tear in tissue. (H) black arrows show cutting artifacts. All images were captured with a 20× objective on a Zeiss Axioscan.z1.