Table 2.
qPCR. Gene expression in injured tendons. Target genes are normalized to the stable housekeeping gene Ppp1ca
| Gene | 7D | 21D | ||||
|---|---|---|---|---|---|---|
| 0 mg/kg | 2 mg/kg | 6 mg/kg | 0 mg/kg | 2 mg/kg | 6 mg/kg | |
| Ccl2 | 1.90 ± 0.83 | 1.41 ± 0.65 | 1.33 ± 0.57 | 1.01 ± 0.47 | 0.98 ± 0.47 | 1.02 ± 0.52 |
| Cd11b | 0.15 ± 0.05 | 0.14 ± 0.09 | 0.12 ± 0.03 | 0.10 ± 0.14 | 0.08 ± 0.06 | 0.05 ± 0.02c |
| Cd163 | 0.26 ± 0.09 | 0.21 ± 0.08 | 0.27 ± 0.07 | 0.07 ± 0.03a | 0.05 ± 0.02b | 0.09 ± 0.04c |
| Col1α1 | 196 ± 39.0 | 197 ± 60.0 | 186 ± 33.0 | 125 ± 60.0 | 118 ± 32.0 | 138 ± 57.0 |
| Col3α1 | 410 ± 133 | 447 ± 176 | 353 ± 108 | 252 ± 112 | 316 ± 151 | 374 ± 192 |
| Hmmr | 0.04 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 | 0.02 ± 0.01a | 0.02 ± 0.01 | 0.03 ± 0.01 |
| Hpgds | 0.03 ± 0.03 | 0.03 ± 0.01 | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.02 ± 0.01 |
| Ptgds | ND | ND | ND | ND | ND | ND |
| Ptges | 0.14 ± 0.04 | 0.13 ± 0.04 | 0.14 ± 0.04 | 0.07 ± 0.03 | 0.07 ± 0.02 | 0.09 ± 0.03 |
| Ptgs1 | 0.06 ± 0.02 | 0.07 ± 0.02 | 0.05 ± 0.02 | 0.04 ± 0.03 | 0.05 ± 0.06 | 0.04 ± 0.02 |
| Ptgs2 | 0.04 ± 0.02 | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.01 ± 0.01 | 0.01 ± 0.01 | 0.01 ± 0.00 |
| Scx | 0.03 ± 0.03 | 0.03 ± 0.01 | 0.02 ± 0.01 | 0.02 ± 0.02 | 0.01 ± 0.01 | 0.02 ± 0.01 |
| Tnmd | 0.62 ± 0.43 | 0.40 ± 0.12 | 0.42 ± 0.20 | 0.96 ± 0.63 | 0.58 ± 0.28 | 0.87 ± 0.46 |
Values presented as mean ± SD. Differences between groups were assessed using a Brown–Forsythe test followed by a Benjamini–Kriege–Yekutieli FDR correction (α = .05) to identify post‐hoc differences between groups: a, different (FDR‐adjusted p < .05) from 7D 0 mg/kg; b, different (FDR‐adjusted p < .05) from 7D 2 mg/kg; c, different (FDR‐adjusted p < .05) from 7D 6 mg/kg; d, different (FDR‐adjusted p < .05) from 21D 0 mg/kg; e, different (FDR‐adjusted p < .05) from 21D 2 mg/kg. N = 6 tendons per group. ND, not detected.