Figure 2.

Time course of the experimental protocols. Protocol 1) The implanted probe was perfused with Ringer's solution containing various concentrations of decynium‐22 or corticosterone. Fluoxetine was co‐administered with the highest concentration of decynium‐22 or corticosterone. An aliquot of dialysate was sampled at baseline and 45–60 min into each perfusion period for 15 min. Protocol 2 and 3) Aliquots of dialysate were sampled before, during, and after the coronary occlusion as shown in the timeline. In the protocol 3, the perfusate was changed to the solution containing 100 µM decynium‐22 at 60 min before the baseline sampling