Fig. 5.

MiR-147/BRMS1 axis was involved in the effects of cyclic mechanical stretch on L6 myoblasts. L6 myoblasts were continually stretched for 24 h and co-transfected with mimics negative control and empty vector (miR-NC + VEC), miR-147 mimics and empty vector (miR-147 + VEC), or miR-147 mimics and BRMS1 overexpressing plasmid (miR-147 + BRMS1-OE). a, b The mRNA and protein levels of BRMS1 in these treated L6 myoblasts were analyzed by qRT-PCR and western blot, respectively. c The proliferation of these treated L6 myoblasts was detected by CCK-8 assay. d, e The apoptosis of these treated L6 myoblasts was detected by caspase-3 activity assay and DNA fragmentation assay. f, g The mRNA and protein levels of ER-Stress-related genes: GRP78, CHOP, and ATF4 in these treated L6 myoblasts were detected by qRT-PCR and western blot, respectively. The data represent the mean ± SD from three independent experiments. **P < 0.01 compared with miR-NC + VEC; ^##P < 0.01 compared with miR-147 + VEC. Student’s t test