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. 2019 Nov 22;10:1238. doi: 10.3389/fneur.2019.01238

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Copyright © 2019 Hernandez, Chatlos, Gorse and Lafrenaye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glia do not appear to be membrane disrupted following CFPI. Representative photomicrographs of the lateral neocortex 2 w following CFPI labeled with (A) GFAP (astrocytes), (B) CC-1/APC (oligodendroglia), (C) Iba-1 (microglia), and (D) NG2 (NG2 cells) (far left panel; red). Membrane disrupted cells containing Alexa-488 conjugated dextran (green) are represented in the second panel. The third panel in blue are DAPI labeled nuclei. The last panel is the overlay images. Arrows depicted cells labeled with each representative marker in relation to the membrane disrupted cells (arrowheads). Note that there were no indications of glial cells sustaining membrane disruption at 2 w post-injury. Scale bar = 20 μm.