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. 2019 Nov 28;9:17865. doi: 10.1038/s41598-019-54334-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ploidy and nuclear number of cardiomyocytes is higher in cyanotic congenital heart disease. (a) Representative immunofluorescence image of multinucleated cardiomyocytes in acyanotic and cyanotic congenital heart disease. Scale bars, 50 μm. (b) The mean nuclear number of cardiomyocytes in both acyanotic and cyanotic group (n = 3 each). (c) The percent of multinucleated cardiomyocytes between acyanotic and cyanotic group (n = 3 each). (d) Representative distribution of ploidy of cardiomyocytes. (e) The mean ploidy of cardiomyocytes (n = 3 each). (f) The percent of polyploid cardiomyocytes between acyanotic and cyanotic group (n = 3 each). (g) Representative WGA staining in both acyanotic and cyanotic group. Scale bars, 50 μm. (h) The result of relative area of cardiomyocytes (n = 5 each). (i) The relative length of cardiomyocytes (n = 5 each). (j) Co-immunostaining with anti-pH3 and anti-cTnT antibodies. Results are displayed as a presentative image (left, scale bars, 25 μm) and as a bar chart (right, n = 6 each). (k) Co-immunostaining with anti-Aurora B and anti-cTnT antibodies. Results are displayed as a representative image (left, scale bars, 10 μm) and as a bar chart (right, n = 6 each). (l) Co-immunostaining with anti-mklp2 and anti-cTnT antibodies. Results are displayed as a representative image (left, scale bars, 10 μm) and as a chart (right, n = 10 each). Data is presented as mean ± s.d. *P < 0.05.