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. 2019 Nov 28;9:17865. doi: 10.1038/s41598-019-54334-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Active β-catenin reduce ploidy and nuclear number of neonatal rat cardiomyocytes. (a) Distribution of ploidy (left, n = 5 samples each) and mean ploidy (right, n = 5 samples each) of neonatal rat cardiomyocyte nuclei via flow cytometry. (b) Distribution of ploidy (left, n = 3 samples each) and mean ploidy (right, n = 3 samples each) of NRCMs. (c) Distribution of ploidy (left, n = 12 samples each) and mean ploidy (right, n = 12 samples each) of NRCMs. (d) Distribution of NRCM ploidy (n = 3 samples each). (e) Relative area of NRCMs (n = 5 samples each). (f) The percent of Ki67-positive cardiomyocyte nuclei (left, n = 9 samples each) and Ki67-positive cardiomyocytes (right, n = 9 samples each). (g) The percent of pH3-positive cardiomyocyte nuclei (left, n = 8 samples each) and pH3-positive cardiomyocytes (right, n = 8 samples each). (h) The percent of Aurora B-positive cardiomyocyte nuclei (left, n = 8 samples each) and Aurora B-positive cardiomyocytes (lower right, n = 8 samples each). (i) The percent of mklp2-positive cardiomyocyte nuclei (upper right, n = 8 samples each) and mklp2-positive cardiomyocytes (lower right, n = 8 samples each). Data is presented as mean ± s.d. *P < 0.05.