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. 2019 Nov 28;9:17822. doi: 10.1038/s41598-019-54044-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decrease in fluorescence intensity after chemically-induced abasic site cleavage on substrates with varying number of dU incorporations and as a function of time. The actual cleavage efficiencies correlate with the loss of fluorescence intensity, resulting from DNA substrate cleavage. The decrease in fluorescence intensity was recorded and normalized to that of the control strand (U₀). The normalized intensities, indicated in arbitrary units, were plotted over the exposure time for the chemical treatments. The DNA array was treated under acidic conditions (A), alkaline conditions (B) or by evaporating the surface to dryness (C) and for various exposure times (1, 2, 4, 8, 12 and 20 hours). The substrate strands contained different ratios of dU nucleotides indicated with different colors: U₀ (black), U₁ (red), U₂ (light green), U₃ (yellow), U₄ (blue), U₅ (pink), U₆ (turquoise), U₇ (gray), U₈ (dark red) and U₉ (dark green). Error bars are SD.