Fig. 3.

HDAC3 knockdown (KD) in primary human BM stromal cells derived from newly-diagnosed MM (ND BMSC), and refractory-relapsed MM (RR BMSC) trigger significant MM cell growth inhibition in MM-MSC co-culture setting. a Figure comparing MM1S.Luc proliferation in co-culture with scramble (left) or HDAC3 si-RNA transfected (right) newly diagnosed (ND, black dots) and refractory relapsed (RR, red squares) BMSC respectively. b Light microscopy images (left) showing the co-culture of MM1S.Luc with scramble or HDAC3 KD BMSCs and fluorescent microscopy images (right) showing mCherry-labeled MM1S.Luc cells co-cultured with scramble or HDAC3 KD BMSCs derived from RR MM (RR BMSC #1). c Dot plot panels show the relative proportion of CD138 negative bone marrow mononuclear cells (BMMC, top rectangle) and CD138 positive MM cells (lower oval) over indicated times in an autologous co-culture system of primary cells obtained from a patient with RRMM. Top panels represent scrambled-transfected CD138 negative, bone marrow mononuclear cells (BMMC), lower panels show HDAC3 siRNA-transfected BMMC. d Chart representing fold changes in MM cell proportion over indicated times in scrambled-transfected and HDAC3 siRNA-transfected BMMC co-culture